A key event in the apoptotic process is the opening of the mitochondrial permeability transition pore, an event that is regulated by Bcl-2 family proteins, resulting in the release of several proteins from the mitochondrial intermembrane space. Several of these proteins participate in apoptosis, including cytochrome c, procaspases 2, 3, and 9 and AIF (apoptosis-inducing factor). AIF was shown to cause DNA fragmentation  and chromatin condensation, and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression was shown to prevent the release of AIF from mitochondria, but not to block its apoptogenic activity.
 

This Abmart monoclonal antibody is produced by immunizing mice with a synthetic  peptide (KLH-coupled) corresponding to residues within the carboxy terminus of AIF.
 

AIF (1B7) Mouse  mAb detects endogenous levels of total  AIF protein.
 

Store at -20°C, stable for one year from the date of shipment.
 

67kDa
 

Human, Rat, Monkey, Chinese hamster, Dog
 

Mouse IgG1
 

Use ananti-MOUSE secondary antibody to detect the antibody.
 

Western blotting      1:2000

*For Western blots, incubate membrane with diluted antibody in 5% w/v non-fat dry milk,  1× TBS, 0.05% Tween-20  at 4°C with gentle shaking, overnight.