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| 肺癌系列Lung Cancer-ATP synthase subunit gamma Mouse mAb
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Background
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14.
Gene Name
Isotype
Predicted Molecular Weight
Reactivity
Application
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ATP5C1 was immunoprecipitated from 1mg of 1G2 cells membrane fraction, blotted with M25352 of 10μg. Western blot was performed from the immunoprecipitate using M25352 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: 1G2 cells membrane fraction. Lane2: IP product of 1G2 cells membrane fraction. Predicted band size : 33 KDa Observed band size : 33 KDa Blocking and dilution buffer and concentration:5% milk/TBST.
Source
This Abmart monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human ATP5C1
Storage
Store at -20°C, stable for one year from the date of shipment.
Recommend Antibody Dilutions
Western blotting 1:1000-1:5000
Immunoprecipitation 10 tests
FACS 1:100-1:200
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