Actins are highly conserved proteins that are involved in various types of  cell motility and are ubiquitously expressed in all eukaryotic cells. As an essential component of cell cytoskeleton, actin plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. In plants, actin preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds, and emerging inflorescence. It displays a very high expression level in mature pollen and pollen tubes.

 

1. Huang S, An YQ, McDowell JM,et al The Arabidopsis ACT11 actin gene is strongly expressed in tissues of the emerging inflorescence, pollen, and developing ovules. Plant Mol Biol. 1997,33(1):125-39.

 

This  Abmart  monoclonal  antibody  is  produced  by  immunizing  mice     with Arabidopsis ACT11 full-length protein.

 

Actin (26F7) Mouse mAb for Plants detects endogenous level of Actin protein.

 

Store the product at -20°C. Stable for one year from the date of shipment.

 

Arabidopsis, Oryza sativa, Nicotiana tabacum

 

Mouse IgG2b

 

45 kDa

 

Use an anti-MOUSE secondary antibody to detect the antibody.

Western blotting                               1:2000

* For Western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1× TBS, 0.05% Tween-20 at 4°C with gentle shaking, overnight.