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| 标签Tag-His-Tag mAb (HRP Conjugated)
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Background
His-Tag mAb (HRP Conjugated) is a monoclonal anti-His antibody covalently linked to horseradish peroxidase.
References
1. Maniattis, T., et al. 1982. Molecular Cloning. Cold Spring Laboratory, Cold Spring Harbor, NY.
2. Hochuli, E. 1988. Large-scale chromatography of recombinant proteins. J.Chromatog. 444: 293-302.
Source
This Abmart monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of human GAPDH.
Specificity
GAPDH (3B3) Mouse mAb detects endogenous levels of total GAPDH protein.
Storage
Store at -20°C. Stable for one year from the date of shipment.
Application
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Western blot analysis of his-tagged fusion protein.
Each lane was loaded with 5ng of his-tagged fusion protein.
Lane 1-6: 1: 500, 1: 1000, 1: 2000, 1: 5000, 1: 10000, 1: 20000
Reactivity
Isotype
Application Tested
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