|
Background
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
References
1. Prasher, D.C., et al. 1992. Primary structure of the Aequorea victoria green fluorescent protein. Gene 111: 229-233.
2. Chalfie, M., et al. 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
Source
This Abmart monoclonal antibody is produced by immunizing animals with full length recombinant GFP.
Specificity
GFP-Tag (7G9) Mouse mAb detects GFP and GFP fusion proteins.
Storage
Store at -20°C. Stable for one year from the date of shipment.
Application
-
Western blot analysis of over-expressed GFP-tagged protein in 293T cell lysate, using Abmart GFP-tag (7G9) Mouse mAb. The antibody dilutions are 1:5000 (lane 1) and 1:10000 (lane 2).
Each lane was loaded with 10 μg of cell lysate.
IF analysis of 293T cells transfected with a GFP-tagged protein, using Abmart GFP-Tag (7G9) Mouse mAb at a 1:500 dilution.
IP of extracts from 293T cells transfected (lane 1,2) with a GFP-tagged protein and a mock transfection (lane 3, using the same protein without the GFP tag), using Abmart GFP-Tag (7G9) Mouse mAb and probed on Western blot using the same antibody.
Dilution: 1:50 (1), 1:100 (2), 1:50 (3)
Isotype
Secondary Antibodies
Use an anti-Mouse secondary antibody to detect the 7G9 antibody.
Recommanded Antibody Dilutions
Western blotting 1:5000
Immunofluorescence 1:500
Immunoprecipitation 1:50
ELISA 1:2000
|
