Catalog No: CY5421 Reactivity: Human Mouse Rat
Isotype: Rabbit IgG Applications: WB IHC ICC IP FC
UniProt ID: P05186
All Names: Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme; ALPL;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 57 kDa
WB: 1:5000~1:10000
IHC: 1:50~1:200
ICC: 1:50~1:200
IP: 1:50
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.

Western blot analysis of Alkaline Phosphatase expression in (1)HepG2 cell lysate; (2)JAR cell lysate.