Catalog No: CY5307 Reactivity: Human Mouse Rat
Isotype: Rabbit IgG Applications: WB IHC ICC/IF IP FC
UniProt ID: O43521
All Names: BAM; BIM; BOD; BimL; BimS; BimEL; BIM-beta6; BIM-beta7; BIM-alpha6;BCL2L11;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 22 kDa
WB 1:500~1:1000
IHC 1:50~1:100
ICC/IF 1:50~1:100
IP 1:40
FC 1:50
Bim a pro-apoptotic member of the BCL-2 protein family. Interacts with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and act as an apoptotic activator. Its expression can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role in neuronal and lymphocyte apoptosis. Transgenic studies in the mouse suggested that this protein functions as an essential initiator of the apoptosis in thymocyte-negative selection. Eight alternatively spliced transcript variants of this gene have been reported.

Western blot analysis of Bim expression in A431 cell lysate.