Catalog No: CY5024 Reactivity: Human, Mouse, Rat
Isotype: Rabbit IgG Applications: WB, IHC, ICC, FC, IP
UniProt ID: O95831
All Names: AIFM1; AIF; MGC111425; PDCD8; COXPD6
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 67 kDa
WB: 1:500~1:2000
IHC: 1:50~1:200
ICC: 1:50~1:200
IP: 1:30
FC: 1:30
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.

Western blot analysis of AIF expression in K562 cell
lysate.