Catalog No: CY5051 Reactivity: Human
Isotype: Rabbit IgG Applications: WB IHC ICC/IF IP FC
UniProt ID: P42574
All Names: CASP3; Caspase-3; APOPAIN; CPP32; CPP32B; SCA-1; Caspase3;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 32 kDa
WB 1:500~1:2000
IHC 1:50~1:200
ICC/IF 1:50~1:200
IP 1:50
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADPribose)polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position.

Western blot analysis of Caspase-3 in HEK293 cell
lysate.