Catalog No: CY5658 Reactivity: Human
Isotype: Rabbit IgG Applications: WB IHC ICC/IF IP FC
UniProt ID: O95352
All Names: ATG7; APG7-LIKE; APG7L; DKFZp434N0735; GSA7;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 78 kDa
WB: 1:10000~1:50000
IHC: 1:50~1:200
ICC/IF: 1:50~1:200
IP: 1:20
FC: 1:20
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents(1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

Western blot analysis of ATG7 expression in HepG2 cell
lysate.