Catalog No: CY5459 Reactivity: Human Rat
Isotype: Rabbit IgG Applications: WB IHC ICC/IF
UniProt ID: P12931
All Names: EC 2.7.10.2; p60-Src; c-Src; pp60;c-src;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 60 kDa
WB: 1:500~1:2000
IHC: 1:50~1:200
ICC/IF: 1:50~1:200
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Western blot analysis of Src expression in U87-MG cell
lysate.