Catalog No: AB0037 Reactivity: H, M, R, Mk, Ch, Ze, Fish
Isotype: Rabbit IgG Applications: WB IHC ICC/IF IP FC
UniProt ID: P04406
All Names: aging-associated gene 9 protein; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; MGC88685;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 37 kDa
WB: 1:3000~1:10000
IHC: 1:100~1:500
IF/ICC: 1:100~1:250
IP: 1:50
FC: 1:50
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.

Western blot analysis of (1) Hela, (2)Jurkat whole cell
lysates, (3)Mouse kidney lysates, (4) Mouse spleen
lysates, (5) RAW 264.7 whole cell lysates, (6) Rat brain
lysates with GAPDH Antibody.