Catalog No: CY5985 Reactivity: Human Mouse Rat
Isotype: Rabbit IgG Applications: WB IHC ICC/IF FC
UniProt ID: Q99816
All Names: TSG101; ESCRT-I complex subunit TSG101; Tumor susceptibility gene 10; Tumor susceptibility
protein; VPS23; Tumor susceptibility gene 101; TSG10;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 44 kDa
WB 1:500~1:2000
IHC 1:50~1:200
ICC/IF 1:50~1:200
FC 1:50
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs).
Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of
cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a
negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins
that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous
retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Western blot analysis of TSG101 expression in MCF-7
cell lysate.