Catalog No: CY6850 Reactivity: Human Mouse Rat
Isotype: Rabbit IgG Applications: WB IHC ICC/IF
UniProt ID: P09874
All Names: ADP-ribosyltransferase 1; poly (ADP-ribose) pol; poly [ADP-ribose] polymerase 1; poly[ADPribose]
synthetase 1; PPOL; ADPRT; ADPRT1; pADPRT-1; PARP; PARP-1
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 113,89 kDa
WB 1:500~1:1000
IHC 1:100~1:500
ICC/IF 1:50~1:200
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.

Western blot analysis of PARP expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with staurosporine.