Catalog No: CY5704 Reactivity: Human Mouse Rat
Isotype: Rabbit IgG Applications: WB IHC ICC IP
UniProt ID: Q7Z3C6
All Names: ATG9A; APG9-like 1; Autophagy 9-like 1 protein; Autophagy-related protein 9A; MGD3208;
MATG9; APG9 autophagy 9-like 1; APG9L1; Autophagy related 9A;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 94 kDa
WB: 1:1000~1:2000
IHC: 1:50~1:200
ICC: 1:50~1:200
IP: 1:50
Atg9, one of the Atg proteins identified in yeast, is essential for autophagosome formation (5). There are two
human functional orthologues based on the yeast homolog Atg9p: Atg9A, which has also been identified as
Atg9L1 and mAtg9, and Atg9L2, which was first reported as nitric-oxide synthase 3 antisense (NOS3AS) (6,7).
Atg9A is an integral membrane protein that is required for both the initiation and the expansion of the
autophagosome (6,7). Recruitment of Atg9A to the autophagosomal membrane is dynamic and transient as
Atg9A also cycles between autophagy-related structures known as omegasomes, the trans-Golgi network
(TGN), and endosomes, and at no point becomes a stable component of the autophagosomal membrane
(6,8). The precise regulation of Atg9A trafficking is not fully clarified, yet it is suggested to involve p38
mitogen-activated protein kinase (MAPK)-binding protein p38IP and the Beclin-1-binding protein Bif-1 (9,10).

Western blot analysis of ATG9A expression in HepG2 cell lysate