Catalog No: CY5114 Reactivity: Human
Isotype: Rabbit IgG Applications: WB IHC ICC/IF IP FC
UniProt ID: P54278
All Names: DNA mismatch repair gene; DNA mismatch repair protein PMS2; HNPCC4; PMS1 protein
homolog 2; PMS2 postmeiotic segregation increased 2; PMS2 postmeiotic segregation
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 110 kDa
WB: 1:500~1:2000
IHC: 1:50~1:200
ICC/IF: 1:50~1:200
IP: 1:50
Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR.Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.

Western blot analysis of PMS2 in (1) Jurkat cell lysate;(2) HeLa cell lysate.