Catalog No: CY5013 Reactivity: Mouse, Rat, Human
Isotype: Rabbit IgG Applications: WB,IHC
UniProt ID: P84022
All Names: JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against
decapentaplegic homolog 3; SMAD 3; Smad 3;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 58 kDa
WB: 1:500~1:2000
IHC 1:50-1:200
Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated
Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In
mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

Western blot analysis of Smad3 expression in (1)Jurkat
cell lysate; (2)Rat liver tissue lysate.