Activation of extracellular signal-regulated  kinase (ERK) or mitogen-activated protein  kinase  by MEK (mitogen-activated protein kinase or extracellular signal-regulated kinase kinase) is an essential event in the mitogenic growth factor-induced signal transduction pathway. 
Phosphorylation of MEKs correlates with their ability to phosphorylate and activate ErKs. MEK1 and MEK2 can also  be activated by autophosphorylation. Lipopolysaccharide activates many of the MAPK family members of the immediate upstream MAPK acti- vator  MEK1, MEK2, and MEK3. In plants, MEK can phosphorylate and activate MAPK, and that Tyr phosphorylation is critical for the catalytic activity  of MAPK in plants.
 

This Abmart polyclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to human MEK1.
 

MEK1 Antibody detects endogenous levels of total MEK1 protein.
 

Store at -20°C, stable for one year from the date of shipment.

45kDa
 

Human, Mouse, Rat, Monkey, Chinese Hamster, Dog
 

Rabbit IgG
 

Use an anti-Rabbit secondary antibody to detect the antibody.
 

Western blotting   1:1000

* For Western blots, incubate membrane with diluted antibody in 5% w/v non-fat dry milk, 1× TBS, 0.05% Tween-20 at 4°C with gentle shaking, overnight.