PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

 

1. Kelman, Z. and O'Donnell, M. (1995) Nucleic Acids Res. 23, 3613-3620.

2. Krishna, T. S. et al. (1994) Cell 79, 1233-1243.

3. Maga, G. and Hubscher, U. (2003) J. Cell Sci. 116, 3051-3060.

 

PCNA polyclonal antibody is produced by immunizing rabbit with a synthetic peptide corresponding to the internal residues of human PCNA.

 

PCNA antibody detects endogenous levels of total PCNA protein.

 

Store at -20°C. Stable for one year from the date of shipment.

 

H, Mk, M, ChHm, Dg, R

 

Rabbit IgG

 

29 kDa

 

WB                 1: 5000-20000

 

Use an anti-RABBIT secondary antibody.