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Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
Source
This Abmart monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human ACTN1
Protein Name
UniProt
Aliases
Actinin; actinin 1 smooth muscle; actinin, alpha 1; alpha 1; alpha actinin 1a; alpha-actinin 1; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; F-actin cross-linking protein; FLJ40884; non-muscle alpha-actinin 1; Non-muscle alpha-actinin-1; 3110023F10Rik; ACTN1; Actn1a; BDPLT15; BOS_11045
Application
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ACTN1 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25063 of 10μg. Western blot was performed from the immunopre-cipitate using M25063 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction. Predicted band size : 103 KDa Observed band size : 103 KDa Blocking and dilution buffer and concentration:5% milk/TBST
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling ACTN1 with M25063 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Recommend Antibody Dilutions
Western blotting 1:1000-1:5000
Immunoprecipitation 10 tests
FACS 1:100-1:200
Predicted Molecular Weight
Storage
Store at -20°C, stable for one year from the date of shipment.
Reactivity
Isotype
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