|
Background
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.
Source
This Abmart monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human
UniProt
Recommend Antibody Dilutions
Western blotting 1:1000-1:5000
Immunoprecipitation 10 tests
FACS 1:100-1:200
Predicted Molecular Weight
Application
-
All lanes : ATPA Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 59 KDa Observed band size : 59 KDa Blocking/Dilution buffer : 1× TBST.
ATPA was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25010 of 10μg. Western blot was performed from the immunoprecipitate using M25010 at 1/2000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2:IP product of Jurkat cells membrane fraction. Blocking and dilution buffer and concentration:5% milk/TBST.
-
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling ATPA with M25010 at 1/200 dilution (green) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Reactivity
Isotype
Aliases
alpha subunit; ATP synthase; ATP synthase alpha chain; ATP synthase alpha chain, mitochondrial; ATP synthase alpha subunit; ATP synthase subunit alpha; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; ATP5A; ATP5AL2; ATPM; cardiac muscle; epididymis secretory sperm binding protein Li 123m; H+ transporting; hATP1; iso; isoform 1; mitochondrial; mitochondrial ATP synthase alpha subunit; mitochondrial ATP synthetase, oligomycin-resistant; mitochondrial F1 complex; mitochondrial H+-ATP synthase alpha subunit; modifier of Min 2; MOM2; OMR; ORM;AI035633; AL022851; AL023067; ATP5A; ATP5A1; ATP5A2; ATP5AL2; ATPM; BOS_22238; COXPD22; D18Ertd206e; hATP1; HEL-S-123m; MC5DN4; MOM2; OMR; ORM; zgc:154103
|
