Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.

1. Barber, R.D. et al. 2005. GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol. Genomics 21, 389-95.2.

2. Hara, M.R. and Snyder, S.H. 2006. Nitric oxide-GAPDH-Siah: a novel cell death cascade. Cell Mol. Neurobiol. 26,527-38.

3. Zheng, L. et al. 2003. S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component. Cell 114, 255-66.

4. Bae, B.l. et al. 2006. Mutant huntingtin: nuclear translocation and cytotoxicity mediated by GAPDH. Proc. Natl. Acad. Sci. USA 103_f 3405-9.

5. Wang, Q. et al. 2005. Proteomic analysis of neurofibrillary tangles in Alzheimer disease identifies GAPDH as a detergent-insoluble paired helical filament tau binding protein. FASEB J. 19,869-71.

This Abmart monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-co叩led) corresponding to the carboxy terminus of human GAPDH.

GAPDH (3B3) Mouse mAb detects endogenous levels of total GAPDH protein. 

Store at -20°C. Stable for one year from the date of shipment.

 

Human

Mouse IgG1

 

Use an anti-MOUSE secondary antibody to detect the 3B3 antibody.

 

Western blotting                                   1:5000

Immunofluorescence                              1:500

For Western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, lx TBS, 0.05% Tween-20 at 4°C with gentle shaking, overnight.