Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase
(GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.

1.Maniatis, T., Fritsch, E.F. and Sambrook, J. 1982. Molecular Cloning- A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
2. Smith, D.B. and Johnson, K.S. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with Glutathione S-transferase. Gene 67: 31-40.
3. Crabb, B.S. and Studdert, M.J. 1995. Expression of small regions of equine herpesvirus 1 glycoprotein C in Escherichia coli. Vet. Microbiol. 46: 181-191.
4. Soler, D., Nomizu, T., Brown, W.E., Shibata, Y. and Auld, D.S. 1995. Matrilysin: expression, purification, and characterization. J. Protein Chem.14: 511-520.

This Abmart monoclonal antibody is produced by immunizing animals with GST.

GST-Tag (12G8) Mouse mAb detects GST and GST fusion proteins.

Store at -20°C. Stable for one year from the date of shipment.

All

Mouse IgG1
 

Use an anti-MOUSE secondary antibody to detect the 12G8 antibody.

Western blotting                1:5000
Immunofluorescence        1:2000
Immunoprecipitation          1:100
ELISA                                1:2000