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Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
References
Munro, S. and Pelham, H.R. 1984. Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp 70. EMBO J. 3, 3087-3093.
Source
This Abmart monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
Specificity
Myc-Tag (19C2) Mouse mAb detects over-expressed or recombinant proteins containing the Myc epitope tag.
Storage
Store at -20°C. Stable for one year from the date of shipment.
Application
Reactivity
Isotype
Secondary Antibodies
Use an anti-Mouse secondary antibody to detect the 19C2 antibody.
Recommanded Antibody Dilutions
Western blotting 1:5000
Immunofluorescence 1:2000
Immunoprecipitation 1:200
ELISA 1:2000
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