Background
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
Source
This Abmart monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human Hsp60
UniProt
Recommend Antibody Dilutions
Western blotting 1:1000-1:5000
Immunoprecipitation 10 tests
FACS 1:100-1:200
Predicted Molecular Weight
Application
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All lanes : Ch60 Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 61 KDa Observed band size : 61 KDa Blocking/Dilution buffer : 1× TBST
Ch60 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25002 of 10μg. Western blot was performed from the immunoprecipitate using M25002 at 1/2000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction. Blocking and dilution buffer and concentration:5% milk/TBST.
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Ch60 with M25002 at 1/200 dilution (green) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Storage
Store at -20°C, stable for one year from the date of shipment.
Reactivity
Isotype
Aliases
60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; Chaperonin 60; CPN60; GROEL; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 60 (liver); heat shock protein 65; heat shock protein, 60 kDa; HLD4; HSP-60; HSP-65; HSP65; HuCHA60; mitochondrial; Mitochondrial matrix protein P1; Mytonin Chaperone 60; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; SPG13; 60kDa; CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HSPD1; Hspd1-30p; HuCHA60; SPG13
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