Catalog No: CY5112 Reactivity: Human Mouse Rat
Isotype: Rabbit IgG Applications: WB IHC ICC/IF IP FC
UniProt ID: P00533
All Names: EC 2.7.10.1; ERBB1; Epidermal growth factor receptor precursor; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 175 kDa
WB: 1:5000~1:10000
IHC: 1: 50~1:200
ICC/IF: 1:50~1:200
IP: 1:50
EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. . A single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans),activation of various downstream signaling molecules and lysosomal degradation. Can be phosphorylated and activated by Src. Activated EGFR binds the SH2 domain of phospholipase C-gamma (PLC-gamma),activating PLC-gamma-mediated downstream signaling. Phosphorylated EGFR binds Cbl, leading to its ubiquitination and degradation.

Western blot analysis of EGFR in HeLa cell lysate.