Catalog No: CY6855 Reactivity: Human
Isotype: Rabbit IgG Applications: WB ICC/IF FC
UniProt ID: O95644
All Names: cytoplasmic 1; NF ATc; NF ATc1; NF-ATc; NF-ATc1; NFAC1; NFAT 2; NFAT transcription
complex cytosolic component; NFAT2; NFATC 1; NFATc; NFATc1; Nuclear factor of activated T cells
cytoplasmic 1; Nuclear factor of activated T-cells;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 101 kDa
WB 1:500~1:2000
ICC/IF 1:50~1:200
FC 1:50
NFAT proteins are predominantly expressed in cells of the immune system, but are also expressed in skeletal muscle, keratinocytes, and adipocytes, regulating cell differentiation programs in these cells. In resting cells,NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus. Termination of NFAT signaling occurs upon declining calcium concentrations and phosphorylation of NFAT by kinases such as GSK-3 or CK1. Cyclosporin A and FK506 are immunosuppressive drugs that inhibit calcineurin and thus retain NFAT proteins in the cytoplasm.

Western blot analysis of NFAT2 expression in Ramos cell lysate.