Catalog No: CY5059 Reactivity: Human Mouse Rat Hamster
Isotype: Rabbit IgG Applications: WB IHC IP FC
UniProt ID: Q07812
All Names: Apoptosis regulator BAX; BAX; Bcl-2-like protein 4; BCL2-associated X protein; Bcl2-L-4; BCL2L4
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 21 kDa
WB 1:1000~1:2000
IHC 1:50~1:200
IP 1:50
Bax is a key component for cellular induced apoptosis through mitochondrial stress. Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane. Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis.

Western blot analysis of Bax in (1) HeLa cell lysate; (2) RAW264.7 cell lysate.