Catalog No: CY5658 Reactivity: Human
Isotype: Rabbit IgG Applications: WB IHC ICC/IF IP FC
UniProt ID: O95352
All Names: ATG7; APG7-LIKE; APG7L; DKFZp434N0735; GSA7;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 78 kDa
WB: 1:10000~1:50000
IHC: 1:50~1:200
ICC/IF: 1:50~1:200
IP: 1:20
FC: 1:20
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents(1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated witha number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

Western blot analysis of ATG7 expression in HepG2 cell lysate.