Catalog No: CY7052 Reactivity: Human Mouse Rat
Isotype: Rabbit IgG Applications: WB IHC IP
UniProt ID: Q8WYN0
All Names: Apg4a; ATG4A; Atg4al; AUT like 2 cysteine endopeptidase; Autl2; Autophagin 2; Autophagin-2;
Autophagy related 4A cysteine peptidase;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 45kDa
WB 1:500~1:2000
IHC 1:50~1:200
IP 1:50
The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.

Western blot analysis of Atg4A expression in (1) HepG2 cell lysate; (2) Mouse brain lysate.