Catalog No: CY5561 Reactivity: Human Mouse Rat
Isotype: Rabbit IgG Applications: WB IHC ICC/IF IP FC
UniProt ID: P42574
All Names: PKB;RAC;PRKBA;PKB-ALPHA;RAC-ALPHA; AKT1 ; AKT2; AKT3;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 56 kDa
WB: 1:500~1:2000
IHC: 1:50~1:200
ICC/IF: 1:50~1:200
IP: 1:50
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at,Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2,responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin,(mTOR) in a
rapamycin-insensitive complex with rictor and Sin1.
Western blot analysis of AKT1/2/3 expression in A549
cell lysate.
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