Catalog No: CY5416 Reactivity: Human Mouse Rat
Isotype: Rabbit IgG Applications: WB IHC

UniProt ID: P21796
All Names: VDAC1;MGC111064;PORIN;PORIN-31-HL;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 31 kDa
WB: 1:500~1:2000
IHC: 1:50~1:200
Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial
membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the
mitochondria (1). In addition, this channel plays a role in apoptotic signaling. The change in mitochondrial
permeability characteristic of apoptosis is mediated by Bcl-2 family proteins, which bind to VDAC, altering the
channel kinetics (2). Homodimerization of VDAC may be a mechanism for changing mitochondrial
permeability and supporting release of cytochrome c (3). In mammalian cells, there are three VDAC isoforms,
VDAC1, which is the most widely expressed isoform, as well as VDAC2 and VDAC3 (4,5).

Western blot analysis of Calreticulin expression in (1)
HepG2 cell lysate; (2) Jurkat cell lysate.